Hooked on a terpene: investigating the presence of withdrawal in zebrafish (Danio rerio) following cessation of repeated exposure to ß-caryophyllene

Abstract

Many psychological and physical conditions have the potential to be treated by terpenes, a class of phytochemicals produced by various plants. The ongoing legalization of medicinal cannabis has prompted further inquiry into both the beneficial and undesirable effects of terpene exposure. In previous research, the terpene ß-caryophyllene was shown to possess an acute sedative effect in zebrafish at a 4% dose and has been shown to possess therapeutic promise in the treatment of anxiety in mice models. However, there is currently minimal research on the capacity for ß-caryophyllene to inadvertently cause anxiety-like behavior when withdrawn. This study will assess anxiety-like and locomotive variables in zebrafish 48-hours post repeated ß-caryophyllene exposure using the open field exploration and novel object approach tests. ß-caryophyllene will be administered as a 4% dose solution. Doses of ß-caryophyllene will be administered for ten minutes over a seven-day period. 48-hours will lapse prior to the onset of behavioral testing. Data will be collected by March 26th, 2023. We expect the zebrafish to display heightened anxiety-like parameters. These parameters will include increased time spent in the thigmotaxis (outer) zone of the tank, greater total distance travelled, and increased velocity relative to control groups. The results of this research could demonstrate the need for caution if one is utilizing ß-caryophyllene for the long-term treatment of anxiety.

Faculty Mentor: Dr. Trevor Hamilton